HLA-E Monoklonaler Antikörper

HLA-E Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4G3

Kat-Nr. : CL488-66530

Synonyme

DKFZp686P19218, EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

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Galerie der Validierungsdaten

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  WB analysis of Raji using CL488-66530

  Raji cells were subjected to SDS PAGE followed by western blot with CL488-66530 (HLA-E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66530 bindet in WB HLA-E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA-E fusion protein Ag6724
• Vollständiger Name: major histocompatibility complex, class I, E
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC002578
• Gene symbol: HLA-E
• Gene ID (NCBI): 3133
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)