HLA-F Polyklonaler Antikörper

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HLA-F Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14670-1-PBS

Synonyme

HLA-5.4, HLA F antigen, HLA F, HLA CDA12, HLA 5.4

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  WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 14670-1-AP (same clone as 14670-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14670-1-PBS bindet in WB, IHC, ELISA HLA-F und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA-F fusion protein Ag6335
• Vollständiger Name: major histocompatibility complex, class I, F
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC062991
• Gene symbol: HLA-F
• Gene ID (NCBI): 3134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)