HLA-G Monoklonaler Antikörper

HLA-G Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

CloneNo.

1E5A10

Kat-Nr. : 66447-1-Ig

Synonyme

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 66447-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66447-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66447-1-Ig

  Human placenta tissue and A375 cell lines (negtive control) were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 66447-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66447-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 12 publications below
• IF: See 13 publications below

Produktinformation

66447-1-Ig bindet in WB, IHC, IF, ELISA HLA-G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA-G fusion protein Ag10839
• Vollständiger Name: major histocompatibility complex, class I, G
• Berechnetes Molekulargewicht: 338 aa, 38 kDa
• Beobachtetes Molekulargewicht: 33-45 kDa
• GenBank-Zugangsnummer: BC021708
• Gene symbol: HLA-G
• Gene ID (NCBI): 3135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.

Publikationen

Species	Application	Title
human	IHC,IF	Aging Cell; Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.; Authors - Liling Xiong
human	IHC,IF	Oxid Med Cell Longev; Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.; Authors - Xin Chen
human	IF	Mol Ther Nucleic Acids; miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia; Authors - Mingyu Hu
human	IHC	FASEB J; Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible role in preeclampsia.; Authors - Manni Sun
human	IF	Placenta; Downregulation of CLDN1 impairs trophoblast invasion and endovascular trophoblast differentiation in early-onset preeclampsia; Authors - Pingsong Jin
human	IF	Biol Reprod; USP7 prevents recurrent spontaneous abortion by targeting EZH2 to regulate trophoblast proliferation, apoptosis, migration and invasion through the Wnt/β-catenin pathway†; Authors - Mengqi Zhou

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