HLA-G Monoklonaler Antikörper

HLA-G Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E5A10

Kat-Nr. : 66447-1-PBS

Synonyme

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

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  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Human placenta tissue and A375 cell lines (negtive control) were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66447-1-PBS bindet in WB, IHC, ELISA HLA-G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HLA-G fusion protein Ag10839
• Vollständiger Name: major histocompatibility complex, class I, G
• Berechnetes Molekulargewicht: 338 aa, 38 kDa
• Beobachtetes Molekulargewicht: 33-45 kDa
• GenBank-Zugangsnummer: BC021708
• Gene symbol: HLA-G
• Gene ID (NCBI): 3135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.