HLF Polyklonaler Antikörper

HLF Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12870-1-PBS

Synonyme

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  WB analysis of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12870-1-AP (same clone as 12870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12870-1-AP (same clone as 12870-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12870-1-AP (same clone as 12870-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12870-1-AP (same clone as 12870-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12870-1-PBS bindet in WB, IHC, Indirect ELISA HLF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLF fusion protein Ag3533
• Vollständiger Name: hepatic leukemia factor
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33kDa, 66 kDa
• GenBank-Zugangsnummer: BC036093
• Gene symbol: HLF
• Gene ID (NCBI): 3131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330).Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands  33 kDa whereas the 66 kDa could be the dimer form of Hlf.