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HLTF Polyklonaler Antikörper

HLTF Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14286-1-PBS

Synonyme

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A

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  • Western Blot (WB) analysis of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    WB analysis of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using HLTF Polyclonal antibody (14286-1-AP)

    WB analysis of K-562 using 14286-1-AP (same clone as 14286-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    IP experiment of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    IF Staining of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14286-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA HLTF und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HLTF fusion protein Ag5596
Vollständiger Name helicase-like transcription factor
Berechnetes Molekulargewicht 114 kDa
Beobachtetes Molekulargewicht 114 kDa
GenBank-ZugangsnummerBC044659
Gene symbol HLTF
Gene ID (NCBI) 6596
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.