HLTF Rekombinanter Antikörper

HLTF Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251000A4

Kat-Nr. : 86362-2-RR

Synonyme

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A

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  WB analysis using 86362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86362-2-RR (HLTF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86362-2-RR (HLTF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86362-2-RR against Human HLTF were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, COS-7-Zellen, HCT 116-Zellen, HeLa-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86362-2-RR bindet in WB, ELISA HLTF und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HLTF fusion protein Ag5596
• Vollständiger Name: helicase-like transcription factor
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: BC044659
• Gene symbol: HLTF
• Gene ID (NCBI): 6596
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.