HMBOX1 Polyklonaler Antikörper

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• KD/KO Validated

HMBOX1 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16123-1-AP

Synonyme

HMBOX1, HNF1LA, homeobox containing 1, Homeobox containing protein 1, PBHNF

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16123-1-AP

  IP Result of anti-HMBOX1 (IP:16123-1-AP, 4ug; Detection:16123-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

16123-1-AP bindet in WB, IP, IHC, ChIP, ELISA HMBOX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMBOX1 fusion protein Ag9081
• Vollständiger Name: homeobox containing 1
• Berechnetes Molekulargewicht: 420 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC009259
• Gene symbol: HMBOX1
• Gene ID (NCBI): 79618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	ChIP	Mol Ther; Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.; Authors - Chenfei Zhou ; KD Validated
human,rat	WB	Front Cardiovasc Med; Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1.; Authors - Guo-Wei Tu
		Oncol Rep; Knockdown of homeobox containing 1 increases the radiosensitivity of cervical cancer cells through telomere shortening.; Authors - Shuliang Zhou
human	WB	Exp Ther Med; HMBOX1 attenuates LPS-induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF-κB signaling pathway.; Authors - Minyuan Nie
human	WB,IHC	Cell Death Dis; WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner.; Authors - Shijie Chen ; KD Validated
human	WB	J Cell Mol Med; 5,2'-dibromo-2,4',5'-trihydroxydiphenylmethanone attenuates LPS-induced inflammation and ROS production in EA.hy926 cells via HMBOX1 induction.; Authors - Hong-Xia Yuan