HMBS Polyklonaler Antikörper

HMBS Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14797-1-AP

Synonyme

EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D

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  WB analysis using 14797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14797-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14797-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14797-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14797-1-AP

  IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:2000) with HEK-293 cells lysate 1200 ug.

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  IF Staining of MCF-7 using 14797-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14797-1-AP

  Immunofluorescent analysis of Hela cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 14797-1-AP

  Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Raji-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14797-1-AP bindet in WB, IF/ICC, IP, ELISA HMBS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMBS fusion protein Ag6509
• Vollständiger Name: hydroxymethylbilane synthase
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC000520
• Gene symbol: HMBS
• Gene ID (NCBI): 3145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.