HMCN1 Polyklonaler Antikörper

HMCN1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18837-1-PBS

Synonyme

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  WB analysis of mouse skin using 18837-1-AP (same clone as 18837-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18837-1-AP (same clone as 18837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18837-1-AP (same clone as 18837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 18837-1-AP (same clone as 18837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18837-1-AP (same clone as 18837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18837-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HMCN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hemicentin 1
• Berechnetes Molekulargewicht: 613 kDa, 600 kDa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_031935
• Gene symbol: HMCN1
• Gene ID (NCBI): 83872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.