HMG20A Monoklonaler Antikörper

HMG20A Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10F12

Kat-Nr. : 66676-1-PBS

Synonyme

1B10F12, high mobility group 20A, HMG box containing protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1

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  WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66676-1-Ig (same clone as 66676-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66676-1-Ig (same clone as 66676-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66676-1-Ig (same clone as 66676-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66676-1-PBS bindet in WB, IF/ICC, Indirect ELISA HMG20A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HMG20A fusion protein Ag2718
• Vollständiger Name: high-mobility group 20A
• Berechnetes Molekulargewicht: 347 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC021959
• Gene symbol: HMG20A
• Gene ID (NCBI): 10363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation