HMG20B Polyklonaler Antikörper

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• KD/KO Validated

HMG20B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14582-1-AP

Synonyme

BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35, high mobility group 20B

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  WB analysis of HeLa using 14582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14582-1-AP

  WB result of HMG20B antibody (14582-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20B transfected HeLa cells.

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  IP experiment of HeLa using 14582-1-AP

  IP result of anti-HMG20B (IP:14582-1-AP, 4ug; Detection:14582-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IHC staining of human colon cancer using 14582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14582-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

14582-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA HMG20B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMG20B fusion protein Ag6103
• Vollständiger Name: high-mobility group 20B
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002552
• Gene symbol: HMG20B
• Gene ID (NCBI): 10362
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].

Publikationen

Species	Application	Title
human	WB	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther
human	WB	Sci Adv; Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.; Authors - Roberto Ravasio
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
mouse	ChIP	Haematologica; The DNA binding factor Hmg20b is a repressor of erythroid differentiation.; Authors - Esteghamat Fatemehsadat F
human	WB,IP,IHC	Med; Inactivation of TACC2 epigenetically represses CDKN1A and confers sensitivity to CDK inhibitors; Authors - Zhi-Rui Lin
human	WB,IP	Mol Cancer; Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma; Authors - Jing Yang