HMGA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HMGA2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (3)

Kat-Nr. : 20795-1-AP

Synonyme

BABL, high mobility group AT hook 2, HMGA2, HMGI C, HMGIC, LIPO, STQTL9

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Galerie der Validierungsdaten

• 

  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 20795-1-AP

  IP Result of anti-HMGA2 (IP:20795-1-AP, 4ug; Detection:20795-1-AP 1:1000) with NIH/3T3 cells lysate 3200ug.

• 

  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 20795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HMGA2 antibody (20795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C6-Zellen, HCT 116-Zellen, HepG2-Zellen, NCI-H1299-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• CoIP: See 3 publications below

Produktinformation

20795-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA HMGA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGA2 fusion protein Ag14588
• Vollständiger Name: high mobility group AT-hook 2
• Berechnetes Molekulargewicht: 108 aa, 12 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: NM_003483
• Gene symbol: HMGA2
• Gene ID (NCBI): 8091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

Publikationen

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.; Authors - Ri-Xin Chen
mouse, rat	WB	ACS Appl Mater Interfaces; Layered Double Hydroxide Nanoparticles with Osteogenic Effects as miRNA Carriers to Synergistically Promote Osteogenesis of MSCs.; Authors - Li Yang
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	CoIP	J Hematol Oncol; MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC.; Authors - Chenggong Sun
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (07-25-2022)

this is very excellent antibody, I have used and it is working great. I will recumbent this antibody if you want for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Colon tissue and renal cell line

FH

Susmita (Verified Customer) (04-13-2022)

This antibody is working fine for my WB

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500
• Cell Tissue Type: CRC cell line

FH

Iram (Verified Customer) (09-17-2020)

HMGA2 bands are very clear and sharp bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

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