HMGB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HMGB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(320)

Rezensionen (2)

Kat-Nr. : 10829-1-AP

Synonyme

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1

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  WB analysis using 10829-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10829-1-AP

  WB result of HMGB1 antibody (10829-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with negative control and HMGB1 knockout HeLa cells.

• 

  WB analysis of mouse kidney using 10829-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10829-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10829-1-AP (HMGB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10829-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HMGB1 antibody (10829-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Jurkat-Zellen, Mausnierengewebe, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 238 publications below
• IHC: See 56 publications below
• IF: See 89 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 7 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

10829-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA HMGB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGB1 fusion protein Ag1264
• Vollständiger Name: high-mobility group box 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC003378
• Gene symbol: HMGB1
• Gene ID (NCBI): 3146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. HMGB1 was oxidized to Ox-HMGB1 at mild ROS concentrations and finally to Di-HMGB1(58 kDa) at excessive ROS concentrations (PMID: 33461096).

Publikationen

Species	Application	Title
mouse	WB,IF	Cell; Transcriptional regulation by PHGDH drives amyloid pathology in Alzheimer's disease; Authors - Junchen Chen
mouse,human	IHC,IF	ACS Nano; Mitochondria-Targeting Polymer Micelle of Dichloroacetate Induced Pyroptosis to Enhance Osteosarcoma Immunotherapy.; Authors - Jiakang Jin
mouse	IF	ACS Nano; Tumor-Acidity and Bioorthogonal Chemistry-Mediated On-Site Size Transformation Clustered Nanosystem to Overcome Hypoxic Resistance and Enhance Chemoimmunotherapy.; Authors - Kewei Wang
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB,IHC,IF	Sci Adv; One-dimensional nanosonosensitizer boosted multiple branches of immune responses against MHC-deficient immune-evasive urologic tumor; Authors - Wei Yang
mouse	IHC	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

FH

PATRICK (Verified Customer) (12-01-2018)

This antibody worked well. ProteinTech provides antibody specific instructions that were helpful for each of the antibodies we ordered.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PRIMARY ISOLATED ENDOTHELIAL CELLS