HMGB1 Rekombinanter Antikörper

HMGB1 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230182C11

Kat-Nr. : 82973-2-PBS

Synonyme

230182C11, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1

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  WB analysis using 82973-2-RR (same clone as 82973-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82973-2-RR (HMGB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82973-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00311-2

  Sandwich ELISA standard curve of MP00311-2, HMGB1 Recombinant Matched Antibody Pair, PBS Only. 82973-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1264. 82973-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  FC experiment of Jurkat using 82973-2-RR (same clone as 82973-2-PBS)

  (blue)1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human HMGB1 (82973-2-RR, Clone:230182C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82973-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82973-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82973-2-PBS against Human HMGB1 was performed. The affinity constant is 2.48 nM.

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Geprüfte Anwendungen

Produktinformation

82973-2-PBS bindet in Sandwich ELISA, Indirect ELISA HMGB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HMGB1 fusion protein Ag1264
• Vollständiger Name: high-mobility group box 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC003378
• Gene symbol: HMGB1
• Gene ID (NCBI): 3146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.