HMGB2 Polyklonaler Antikörper

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• KD/KO Validated

HMGB2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 14597-1-AP

Synonyme

HMG-2, HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2

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  WB analysis of Jurkat using 14597-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14597-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14597-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 14597-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14597-1-AP

  IP result of anti-HMGB2 (IP:14597-1-AP, 4ug; Detection:14597-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of mouse brain using 14597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14597-1-AP (HMGB2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14597-1-AP (HMGB2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGB2 antibody (14597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HL-60-Zellen, humanes Cerebellum-Gewebe, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IF: See 4 publications below

Produktinformation

14597-1-AP bindet in WB, IF/ICC, IP, ELISA HMGB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGB2 fusion protein Ag6135
• Vollständiger Name: high-mobility group box 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC001063
• Gene symbol: HMGB2
• Gene ID (NCBI): 3148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains (11246022,14871457). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (14871457). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (11262228). HMGB3 is only expressed during embryogenesis (9598312). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (11246022,14871457). Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes (19317908 ,10490593). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE) (19811285 ). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time (20851854).This antibody recognizes the phosphorylation form of HMGB2 protein. The calculated molecular weight of HMGB2 is 24 kDa, but the post-modifiction of HMGB2 is about 33-35 kDa. (PMID: 18218727)

Publikationen

Species	Application	Title
human,mouse		Front Immunol; Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression; Authors - Zhao Guang
mouse	WB	Ecotoxicol Environ Saf; Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance; Authors - Mengting Xu
rat	WB	Environ Pollut; Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.; Authors - Yongli Han
mouse	WB	Chemosphere; Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.; Authors - Yongli Han
human	WB	Cancer Gene Ther; The LncRNA RP11-301G19.1/miR-582-5p/HMGB2 axis modulates the proliferation and apoptosis of multiple myeloma cancer cells via the PI3K/AKT signalling pathway.; Authors - Faming Wang
human	WB	Cell Commun Signal; HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.; Authors - Deyuan Fu ; KD Validated