HMGB2 Rekombinanter Antikörper

HMGB2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240453C11

Kat-Nr. : 83482-4-PBS

Synonyme

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

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  WB analysis using 83482-4-RR (same clone as 83482-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83482-4-RR (HMGB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83482-4-RR (same clone as 83482-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-4-RR, Clone:240453C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83482-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83482-4-RR against Human HMGB2 were performed. The affinity constant is 16.4 nM.

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Geprüfte Anwendungen

Produktinformation

83482-4-PBS bindet in WB, ELISA HMGB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HMGB2 fusion protein Ag6135
• Vollständiger Name: high-mobility group box 2
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001063
• Gene symbol: HMGB2
• Gene ID (NCBI): 3148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.