HMGCL Rekombinanter Antikörper

HMGCL Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242224G10

Kat-Nr. : 84769-1-PBS

Synonyme

242224G10, EC:4.1.3.4, HL, HMG CoA lyase, HMG-CoA lyase

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  WB analysis using 84769-1-RR (same clone as 84769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84769-1-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01555-2

  Cytometric bead array standard curve of MP01555-2, HMGCL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84769-2-PBS. Detection antibody: 84769-1-PBS. Standard: Ag10426. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 84769-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84769-1-RR against Human HMGCL were performed. The affinity constant is 0.408 nM.

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Geprüfte Anwendungen

Produktinformation

84769-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA HMGCL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HMGCL fusion protein Ag10426
• Vollständiger Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Berechnetes Molekulargewicht: 325 aa, 34 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC010570
• Gene symbol: HMGCL
• Gene ID (NCBI): 3155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.