HMGCS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HMGCS1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (3)

Kat-Nr. : 17643-1-AP

Synonyme

HMG CoA synthase, HMGCS, HMGCS1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17643-1-AP

  WB result of HMGCS1 antibody (17643-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGCS1 transfected HepG2 cells.

• 

  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17643-1-AP

  IP Result of anti-HMGCS1 (IP:17643-1-AP, 3ug; Detection:17643-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IF Staining of HepG2 using 17643-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMGCS1 antibody (17643-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HepG2-Zellen, LNCaP-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 28 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

17643-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA HMGCS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGCS1 fusion protein Ag11825
• Vollständiger Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
• Berechnetes Molekulargewicht: 520 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC083514
• Gene symbol: HMGCS1
• Gene ID (NCBI): 3157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis; Authors - Wei Zhou ; KD Validated
human	WB	Nat Cancer; Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism; Authors - Linjie Zhao ; KD Validated
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse,human	IF,WB	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
human	WB	Mol Cell Proteomics; Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.; Authors - Saraon Punit P

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (12-20-2020)

Used for western blotting (1:1000) and IHC (1:200), worked well for me

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

H (Verified Customer) (07-09-2020)

This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and HCT116

HMGCS1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:17643-1-AP)

FH

Boyan (Verified Customer) (07-08-2019)

This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.

• Applications: Western Blot,
• Cell Tissue Type: MEF