HMGN2 Polyklonaler Antikörper

HMGN2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10953-1-PBS

Synonyme

Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2

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  WB analysis of Jurkat using 10953-1-AP (same clone as 10953-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10953-1-AP (same clone as 10953-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10953-1-AP (same clone as 10953-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10953-1-AP (same clone as 10953-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10953-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HMGN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMGN2 fusion protein Ag1401
• Vollständiger Name: high-mobility group nucleosomal binding domain 2
• Berechnetes Molekulargewicht: 9 aa, 3 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC014644
• Gene symbol: HMGN2
• Gene ID (NCBI): 3151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.