HMMR Monoklonaler Antikörper

HMMR Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6B5

Kat-Nr. : 68718-1-Ig

Synonyme

RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein, IHABP, CD168

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  WB analysis using 68718-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68718-1-Ig (HMMR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68718-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR antibody (68718-1-Ig, Clone: 1C6B5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68718-1-Ig bindet in WB, IF/ICC, ELISA HMMR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HMMR fusion protein Ag33723
• Vollständiger Name: hyaluronan-mediated motility receptor (RHAMM)
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: NM_012484
• Gene symbol: HMMR
• Gene ID (NCBI): 3161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)