HMMR-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HMMR-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (1)

Kat-Nr. : 15820-1-AP

Synonyme

HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein

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• 

  WB analysis using 15820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15820-1-AP (HMMR-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15820-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR-Specific antibody (15820-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HepG2 using 15820-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human HMMR-Specific (15820-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C6-Zellen, K-562-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15820-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA HMMR-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hyaluronan-mediated motility receptor (RHAMM)
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: NM_001142556
• Gene symbol: HMMR
• Gene ID (NCBI): 3161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)

Publikationen

Species	Application	Title
mouse	FC	Nature; Tracing haematopoietic stem cell formation at single-cell resolution.; Authors - Fan Zhou
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
rat	IF	Int J Mol Sci; Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat; Authors - Jian-He Lu
human	WB	Int J Biol Sci; Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.; Authors - Deyu Zhang ; KD Validated
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Acta Biochim Biophys Sin (Shanghai); FOXM1 mediates methotrexate resistance in osteosarcoma cells by promoting autophagy; Authors - Luoyang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Heriberto (Verified Customer) (11-28-2018)

Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .

• Applications: Western Blot,
• Primary Antibody Dilution: Try 1:1000, 1:750 and 1:500
• Cell Tissue Type: SN12L1, SN12C and ACHN- Renal carcinoma cell lines

HMMR-Specific Antibody Western Blot, validation (Try 1:1000, 1:750 and 1:500 dilution) in SN12L1, SN12C and ACHN- Renal carcinoma cell lines (Cat no:15820-1-AP)