HMOX2 Polyklonaler Antikörper

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• KD/KO Validated

HMOX2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 14817-1-AP

Synonyme

EC:1.14.14.18, heme oxygenase (decycling) 2, Heme oxygenase 2, Heme oxygenase 2 soluble form, HO 2

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  WB analysis of HepG2 using 14817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 14817-1-AP

  WB result of HMOX2 antibody (14817-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMOX2 transfected hTERT-RPE1 cells.

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  WB analysis of Raji using 14817-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14817-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 14817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14817-1-AP

  IP result of anti-HMOX2 (IP:14817-1-AP, 4ug; Detection:14817-1-AP 1:500) with Raji cells lysate 2000ug.

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  IHC staining of human lymphoma using 14817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14817-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HMOX2 antibody (14817-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14817-1-AP

  Immunofluorescent analysis of Hela cells, using HMOX2 antibody 14817-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, hTERT-RPE1-Zellen, K-562-Zellen, Mausmilzgewebe, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

14817-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HMOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HMOX2 fusion protein Ag6575
• Vollständiger Name: heme oxygenase (decycling) 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002396
• Gene symbol: HMOX2
• Gene ID (NCBI): 3163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.; Authors - Gokhan Unlu
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
human	WB	J Cell Sci; Role of epithelial sodium channels (ENaCs) in endothelial function.; Authors - Dongqing Guo
mouse,human	WB,IHC	Oxid Med Cell Longev; Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.; Authors - László Potor
mouse	WB,IHC	Am J Physiol Gastrointest Liver Physiol; Carbon monoxide produced by HO-1 upregulation is the main factor behind the abnormal motility seen in experimental ulcerative colitis in mice; Authors - Mengchao Zhao
rat	WB	Cells; Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions.; Authors - Katarzyna Magierowska