HN1 Polyklonaler Antikörper

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• KD/KO Validated

HN1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14914-1-AP

Synonyme

Androgen regulated protein 2, ARM2, HN1, HN1A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14914-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14914-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14914-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14914-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14914-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14914-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14914-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14914-1-AP

  IP Result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

• 

  IHC staining of human ovary tumor using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Hodengewebe, LNCaP-Zellen, Maushodengewebe, Maus-Kolongewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14914-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA HN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HN1 fusion protein Ag6712
• Vollständiger Name: hematological and neurological expressed 1
• Berechnetes Molekulargewicht: 11 kDa, 16 kDa, 19 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC001420
• Gene symbol: HN1
• Gene ID (NCBI): 51155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

Publikationen

Species	Application	Title
human	WB,CoIP	Cancer Lett; HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.; Authors - Zongfu Pan ; KO Validated
human	WB,IHC	Kaohsiung J Med Sci; Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.; Authors - Jia-Jie Chen ; KD Validated
human	WB	Chem Biol Drug Des; Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo; Authors - Qiusheng Guo
human	WB,IF	Cancer Lett; Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer; Authors - Zongfu Pan