HN1 Polyklonaler Antikörper

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HN1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14914-1-PBS

Synonyme

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  WB analysis of HeLa using 14914-1-AP (same clone as 14914-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 14914-1-AP (same clone as 14914-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 14914-1-AP (same clone as 14914-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 14914-1-AP (same clone as 14914-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 14914-1-AP (same clone as 14914-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 14914-1-AP (same clone as 14914-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 14914-1-AP (same clone as 14914-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14914-1-AP (same clone as 14914-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 14914-1-AP (same clone as 14914-1-PBS)

  IP result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14914-1-AP (same clone as 14914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14914-1-AP (same clone as 14914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14914-1-PBS bindet in WB, IHC, IP, Indirect ELISA HN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HN1 fusion protein Ag6712
• Vollständiger Name: hematological and neurological expressed 1
• Berechnetes Molekulargewicht: 11 kDa, 16 kDa, 19 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC001420
• Gene symbol: HN1
• Gene ID (NCBI): 51155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.