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HN1L Polyklonaler Antikörper

HN1L Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16285-1-PBS

Synonyme

C16orf34, Hematological and neurological expressed 1-like protein, HN1-like protein, JPT2, Jupiter microtubule associated homolog 2

Filter:
  • Western Blot (WB) analysis of various lysates using HN1L Polyclonal antibody (16285-1-AP)

    WB analysis using 16285-1-AP (same clone as 16285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HN1L Polyclonal antibody (16285-1-AP)

    IHC staining of human stomach using 16285-1-AP (same clone as 16285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16285-1-AP (HN1L antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HN1L Polyclonal antibody (16285-1-AP)

    IF Staining of A431 using 16285-1-AP (same clone as 16285-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16285-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HN1L und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HN1L fusion protein Ag9400
Vollständiger Name hematological and neurological expressed 1-like
Berechnetes Molekulargewicht 190 aa, 20 kDa
Beobachtetes Molekulargewicht20-25 kDa
GenBank-ZugangsnummerBC014438
Gene symbol HN1L
Gene ID (NCBI) 90861
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).