HNF1B Polyklonaler Antikörper

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• KD/KO Validated

HNF1B Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12533-1-PBS

Synonyme

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

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  WB analysis of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 12533-1-AP (same clone as 12533-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12533-1-AP (same clone as 12533-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12533-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HNF1B und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNF1B fusion protein Ag3232
• Vollständiger Name: HNF1 homeobox B
• Berechnetes Molekulargewicht: 557 aa, 61 kDa
• Beobachtetes Molekulargewicht: 58-65 kDa
• GenBank-Zugangsnummer: BC017714
• Gene symbol: HNF1B
• Gene ID (NCBI): 6928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.