HNF1B Rekombinanter Antikörper

HNF1B Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242120A9

Kat-Nr. : 84684-4-RR

Synonyme

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

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  WB analysis of MDCK using 84684-4-RR

  MDCK cells were subjected to SDS PAGE followed by western blot with 84684-4-RR (HNF1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84684-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84684-4-RR against Human HNF1B were performed. The affinity constant is 0.232 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84684-4-RR bindet in WB, ELISA HNF1B und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNF1B fusion protein Ag3232
• Vollständiger Name: HNF1 homeobox B
• Berechnetes Molekulargewicht: 557 aa, 61 kDa
• Beobachtetes Molekulargewicht: 58-65 kDa
• GenBank-Zugangsnummer: BC017714
• Gene symbol: HNF1B
• Gene ID (NCBI): 6928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.