HNF1B Rekombinanter Antikörper

HNF1B Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242120H8

Kat-Nr. : 84684-5-RR

Synonyme

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

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  IF Staining of HepG2 using 84684-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Caco-2 using 84684-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Caco-2 using 84684-5-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84684-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in FC (Intra): Caco-2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84684-5-RR bindet in IF/ICC, FC (Intra), ELISA HNF1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNF1B fusion protein Ag3232
• Vollständiger Name: HNF1 homeobox B
• Berechnetes Molekulargewicht: 557 aa, 61 kDa
• GenBank-Zugangsnummer: BC017714
• Gene symbol: HNF1B
• Gene ID (NCBI): 6928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.