HNRNPA1 Monoklonaler Antikörper

HNRNPA1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5A4

Kat-Nr. : 67844-1-PBS

Synonyme

hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein, Helix destabilizing protein

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  WB analysis using 67844-1-Ig (same clone as 67844-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67844-1-Ig (same clone as 67844-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67844-1-Ig (same clone as 67844-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67844-1-Ig (same clone as 67844-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (67844-1-Ig, Clone: 1A5A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67844-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HNRNPA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HNRNPA1 fusion protein Ag17415
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009600
• Gene symbol: HNRNPA1
• Gene ID (NCBI): 3178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.