HNRNPA2B1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPA2B1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(71)

Kat-Nr. : 14813-1-AP

Synonyme

FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPB1, HNRPA2

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14813-1-AP

  WB result of HNRNPA2B1 antibody (14813-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPA2B1 transfected A549 cells.

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14813-1-AP (HNRNPA2B1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HeLa-Zellen, HL-60-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 54 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below

Produktinformation

14813-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA HNRNPA2B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPA2B1 fusion protein Ag6563
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 36 kDa and 38 kDa
• GenBank-Zugangsnummer: BC000506
• Gene symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu
human	IF	Mol Cancer; The therapeutically actionable long non-coding RNA 'T-RECS' is essential to cancer cells' survival in NRAS/MAPK-driven melanoma; Authors - Valentin Feichtenschlager
mouse	WB	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IF	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
human	WB,IF,CoIP	Theranostics; LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance; Authors - Hui-Fang Hu ; KD Validated