HNRNPA2B1 Rekombinanter Antikörper

HNRNPA2B1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240831E3

Kat-Nr. : 83773-7-RR

Synonyme

HNRPA2, HNRNPB1, HNRNPA2, hnRNP A2/B1, FLJ22720

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  WB analysis using 83773-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83773-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83773-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83773-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (83773-7-RR, Clone: 240831E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83773-7-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPA2B1 Recombinant antibody (83773-7-RR, Clone:240831E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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  Affinity and Kinetic Characterization of 83773-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83773-7-RR against Human HNRNPA2B1 were performed. The affinity constant is 5.04 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83773-7-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA HNRNPA2B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNRNPA2B1 fusion protein Ag6563
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 36 kDa, 38 kDa
• GenBank-Zugangsnummer: BC000506
• Gene symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.