HNRNPA2B1 Rekombinanter Antikörper

HNRNPA2B1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

V3H6F7F2

Kat-Nr. : 86713-1-MR

Synonyme

FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPB1, HNRPA2

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  WB analysis using 86713-1-MR

  Various lysates were subjected to SDS PAGE followed by western blot with 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86713-1-MR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 86713-1-MR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86713-1-MR (HNRNPA2B1 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 86713-1-MR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red).

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  IF Staining of HeLa using 86713-1-MR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (86713-1-MR, Clone: V3H6F7F2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:7500-1:30000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86713-1-MR bindet in WB, IHC, IF/ICC, ELISA HNRNPA2B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNRNPA2B1 fusion protein Ag6699
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 34, 36 kDa
• GenBank-Zugangsnummer: BC000506
• Gene symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.