HNRNPD Polyklonaler Antikörper

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• KD/KO Validated

HNRNPD Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 12770-1-AP

Synonyme

AUF1, AUF1A, AU-rich element RNA-binding protein 1, hnRNP D0, hnRNPD0

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  WB analysis using 12770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12770-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12770-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12770-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12770-1-AP (HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP (HNRNPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 12770-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12770-1-AP (HNRNPD Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 12770-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (12770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

12770-1-AP bindet in WB, IHC, IF/ICC, ELISA HNRNPD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPD fusion protein Ag3458
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
• Berechnetes Molekulargewicht: 355 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC026015
• Gene symbol: HNRNPD
• Gene ID (NCBI): 3184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

Publikationen

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	IHC	Brain; Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions.; Authors - Johnathan Cooper-Knock
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	EMBO Rep; Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates.; Authors - Johan Aarum