HNRNPF Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPF Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2E7A4

Kat-Nr. : 67701-1-Ig

Synonyme

2E7A4, Heterogeneous nuclear ribonucleoprotein F, N-terminally processed, hnRNP F, HNRPF, mcs94 1

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  WB analysis using 67701-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67701-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67701-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• RIP: See 1 publications below

Produktinformation

67701-1-Ig bindet in WB, IF/ICC, RIP, ELISA HNRNPF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HNRNPF fusion protein Ag7128
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein F
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001432
• Gene symbol: HNRNPF
• Gene ID (NCBI): 3185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; mTOR inhibition reprograms cellular proteostasis by regulating eIF3D-mediated selective mRNA translation and promotes cell phenotype switching; Authors - Sejeong Shin ; KD Validated
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	RNA Biol; hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.; Authors - Tanzina Tanu ; KD Validated
mouse	WB	Asian J Androl; Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis; Authors - Shui-Qiao Q Yuan
human	WB	J Hepatol; Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD; Authors - Long Yang
human	WB	Acta Biochim Biophys Sin (Shanghai); SUN5, a testis-specific nuclear membrane protein, participates in recruitment and export of nuclear mRNA in spermatogenesis; Authors - Xiyi He