HNRNPH1 Polyklonaler Antikörper

HNRNPH1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 14774-1-AP

Synonyme

Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1

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  WB analysis using 14774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14774-1-AP (HNRNPH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryonic stem cells using 14774-1-AP

  WB result from Dr. Corbit Kevin. Mouse embryonic stem cell were used to test the antibody 14774-1-AP.

• 

  IHC staining of human cervical cancer using 14774-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14774-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14774-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, embryonale Stammzellen der Maus, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14774-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA HNRNPH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPH1 fusion protein Ag6506
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein H1 (H)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49-50 kDa
• GenBank-Zugangsnummer: BC001348
• Gene symbol: HNRNPH1
• Gene ID (NCBI): 3187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen
mouse	WB	FASEB J; 5' UTR variants in the quantitative trait gene Hnrnph1 support reduced 5' UTR usage and hnRNP H protein as a molecular mechanism underlying reduced methamphetamine sensitivity.; Authors - Qiu T Ruan
mouse	IF	Biomedicines; C9orf72 Hexanucleotide Repeat Expansion-Related Neuropathology Is Attenuated by Nasal Rifampicin in Mice.; Authors - Yukari Hatanaka

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Nuclear staining - acceptable (a bit on a weak side). WB with semi-dry transfer 4% milk blocking in TBST, 1:1000 dilution - good signal.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa and SH-SY5Y cells/human

HNRNPH1 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in HeLa and SH-SY5Y cells/human (Cat no:14774-1-AP)