HNRNPH1 Monoklonaler Antikörper

HNRNPH1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A6C2

Kat-Nr. : 67375-1-PBS

Synonyme

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

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  WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67375-1-Ig (same clone as 67375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67375-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HNRNPH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HNRNPH1 fusion protein Ag6603
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein H1 (H)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49-50 kDa
• GenBank-Zugangsnummer: BC001348
• Gene symbol: HNRNPH1
• Gene ID (NCBI): 3187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].