HNRNPL Polyklonaler Antikörper

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• KD/KO Validated

HNRNPL Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18354-1-PBS

Synonyme

P/OKcl.14, HNRPL, hnRNP L

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  WB analysis using 18354-1-AP (same clone as 18354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18354-1-AP (same clone as 18354-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IP experiment of BxPC-3 using 18354-1-AP (same clone as 18354-1-PBS)

  IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18354-1-AP (same clone as 18354-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPL antibody (18354-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18354-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HNRNPL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPL fusion protein Ag13211
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein L
• Berechnetes Molekulargewicht: 572 aa, 62 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC069184
• Gene symbol: HNRNPL
• Gene ID (NCBI): 3191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.