HNRNPL Rekombinanter Antikörper

HNRNPL Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3J22

Kat-Nr. : 82259-1-RR

Synonyme

P/OKcl.14, HNRPL, hnRNP L, 3J22

---

• 

  WB analysis using 82259-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82259-1-RR (HNRNPL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82259-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 82259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82259-1-RR against Human HNRNPL were performed. The affinity constant is 6.54 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, BxPC-3-Zellen, C6-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:19600
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82259-1-RR bindet in WB, IHC, ELISA HNRNPL und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNRNPL fusion protein Ag13211
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein L
• Berechnetes Molekulargewicht: 572 aa, 62 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC069184
• Gene symbol: HNRNPL
• Gene ID (NCBI): 3191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.