HNRNPM Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HNRNPM Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 26897-1-AP

Synonyme

hnRNP M, HNRNPM4, HNRPM, HNRPM4, HTGR1

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  WB analysis of COLO 320 using 26897-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26897-1-AP (HNRNPM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26897-1-AP

  WB result of HNRNPM antibody (26897-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPM transfected MCF-7 cells.

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  WB analysis using 26897-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26897-1-AP (HNRNPM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 26897-1-AP

  IP and Co-IP result of anti-HNRNPM (IP:26897-1-AP, 4ug); Detection antibodies: HNRNPM 26897-1-AP 1:3000 and HNRNPC 11760-1-AP 1:3000) with HEK-293T cells lysate 1840 ug.

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  IHC staining of human tonsillitis using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (26897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 26897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (26897-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, human colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

26897-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HNRNPM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPM fusion protein Ag25474
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein M
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 73-77 kDa
• GenBank-Zugangsnummer: BC000138
• Gene symbol: HNRNPM
• Gene ID (NCBI): 4670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPM, also named as NAGR1, is a 730 amino acid protein, which localizes in nucleus. HNRNPM as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. HNRNPM is Involved in splicing. HNRNPM acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. HNRNPM exists two isoforms with MV 77 kDa and 73 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
human	WB	Proc Natl Acad Sci U S A; CircURI1 interacts with hnRNPM to inhibit metastasis by modulating alternative splicing in gastric cancer.; Authors - Xiaolin Wang
human	WB	J Exp Clin Cancer Res; PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.; Authors - Pingfu Hou
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang
human	WB,IP	Sci Rep; Unraveling the independent role of METTL3 in m6A modification and tumor progression in esophageal squamous cell carcinoma; Authors - Bin Du

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa/human