HNRNPR Polyklonaler Antikörper

HNRNPR Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29980-1-AP

Synonyme

FLJ25714, hnRNP R, HNRNPR, HNRPR

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  WB analysis using 29980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29980-1-AP (HNRNPR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29980-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29980-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29980-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 29980-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HNRNPR antibody (29980-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

29980-1-AP bindet in WB, IHC, IF/ICC, CoIP, RIP, ELISA HNRNPR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPR fusion protein Ag30339
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein R
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 65-82 kDa
• GenBank-Zugangsnummer: BC001449
• Gene symbol: HNRNPR
• Gene ID (NCBI): 10236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

Publikationen

Species	Application	Title
human	WB	J Med Genet; HnRNPR strongly represses splicing of a critical exon associated with spinal muscular atrophy through binding to an exonic AU-rich element; Authors - Tao Jiang
human	WB,RIP	Biochim Biophys Acta Mol Basis Dis; HNRNPA2B1 and HNRNPR stabilize ASCL1 in an m6A-dependent manner to promote neuroblastoma progression; Authors - Ting Hu
human	WB,RIP,IF,CoIP	Int J Biol Sci; Mannose Inhibits NSCLC Growth and Inflammatory Microenvironment by Regulating Gut Microbiota and Targeting OGT/hnRNP R/JUN/IL-8 Axis; Authors - Haoyi Jin