HNRNPR Polyklonaler Antikörper

HNRNPR Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29980-1-PBS

Synonyme

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  WB analysis using 29980-1-AP (same clone as 29980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29980-1-AP (HNRNPR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29980-1-AP (same clone as 29980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29980-1-AP (same clone as 29980-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HNRNPR antibody (29980-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29980-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29980-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HNRNPR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPR fusion protein Ag30339
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein R
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 65-82 kDa
• GenBank-Zugangsnummer: BC001449
• Gene symbol: HNRNPR
• Gene ID (NCBI): 10236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)