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HNRNPR Rekombinanter Antikörper

HNRNPR Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242734C8

Kat-Nr. : 85078-1-PBS

Synonyme

242734C8, hnRNP R, HNRPR

Filter:
  • Western Blot (WB) analysis of various lysates using HNRNPR Recombinant antibody (85078-1-RR)

    WB analysis using 85078-1-RR (same clone as 85078-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85078-1-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPR Recombinant antibody (85078-1-RR)

    IF Staining of HepG2 using 85078-1-RR (same clone as 85078-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPR antibody (85078-1-RR, Clone: 242734C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85078-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85078-1-RR

    Affinity and Kinetic Characterization of 85078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85078-1-RR against Human HNRNPR were performed. The affinity constant is 0.545 nM.

Geprüfte Anwendungen

Produktinformation

85078-1-PBS bindet in WB, IF/ICC, Indirect ELISA HNRNPR und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HNRNPR fusion protein Ag30339
Vollständiger Name heterogeneous nuclear ribonucleoprotein R
Berechnetes Molekulargewicht 71 kDa
Beobachtetes Molekulargewicht65-82 kDa
GenBank-ZugangsnummerBC001449
Gene symbol HNRNPR
Gene ID (NCBI) 10236
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)