HNRNPR Rekombinanter Antikörper

HNRNPR Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242734C8

Kat-Nr. : 85078-1-RR

Synonyme

HNRPR, hnRNP R, 242734C8

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  WB analysis using 85078-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85078-1-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85078-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPR antibody (85078-1-RR, Clone: 242734C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85078-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85078-1-RR against Human HNRNPR were performed. The affinity constant is 0.545 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85078-1-RR bindet in WB, IF/ICC, ELISA HNRNPR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HNRNPR fusion protein Ag30339
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein R
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 65-82 kDa
• GenBank-Zugangsnummer: BC001449
• Gene symbol: HNRNPR
• Gene ID (NCBI): 10236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)