HNRNPU Polyklonaler Antikörper

HNRNPU Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 14599-1-AP

Synonyme

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

---

Galerie der Validierungsdaten

• 

  WB analysis using 14599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14599-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14599-1-AP (HNRNPU antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14599-1-AP

  IP Result of anti-HNRNPU (IP:14599-1-AP, 4ug; Detection:14599-1-AP 1:800) with HeLa cells lysate 2400ug.

• 

  IF Staining of HeLa using 14599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (14599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslungengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Produktinformation

14599-1-AP bindet in WB, RIP, IP, IF, ELISA HNRNPU und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HNRNPU fusion protein Ag6141
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• Berechnetes Molekulargewicht: 825 aa, 91 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC003621
• Gene symbol: HNRNPU
• Gene ID (NCBI): 3192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Publikationen

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB,IP	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li
human	WB,RIP	Cancer Res; DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.; Authors - Songge Xing
human	WB	Elife; Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction; Authors - Amy E Spens
human	WB	Hum Mol Genet; Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs.; Authors - Hiroaki Suzuki