HO-1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

HO-1 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2M6

Kat-Nr. : 81281-1-PBS

Synonyme

HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

---

• 

  WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81281-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

81281-1-PBS bindet in WB, IP, Indirect ELISA HO-1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HO-1 fusion protein Ag1190
• Vollständiger Name: heme oxygenase (decycling) 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001491
• Gene symbol: HO-1
• Gene ID (NCBI): 3162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).