HO-1/Hmox1 Polyklonaler Antikörper

HO-1/Hmox1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31253-1-AP

Synonyme

Hmox1, HO-1, D8Wsu38e, heme oxygenase (decycling) 1, Hemox

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  WB analysis using 31253-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 31253-1-AP (HO-1/Hmox1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31253-1-AP (HO-1/Hmox1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31253-1-AP bindet in WB, ELISA HO-1/Hmox1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HO-1/Hmox1 fusion protein Eg0938
• Vollständiger Name: heme oxygenase (decycling) 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_010442.2
• Gene symbol: HO-1
• Gene ID (NCBI): 15368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heme Oxygenase-1 (HO-1) is a stress-inducible enzyme that plays a crucial role in cellular and tissue homeostasis. It is the rate-limiting enzyme responsible for the oxidative degradation of heme, producing carbon monoxide (CO), free iron, and biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These byproducts have significant biological activities, contributing to HO-1's anti-inflammatory, cytoprotective, and immunomodulatory effects. (PMID: 33923744)