HO-1/Hmox1 Rekombinanter Antikörper

HO-1/Hmox1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240236G6

Kat-Nr. : 83291-2-PBS

Synonyme

Hmox1, HO-1, D8Wsu38e, heme oxygenase (decycling) 1, Hemox

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  Sandwich ELISA standard curve of MP00318-3

  Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00318-3

  Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

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  Sample test of MP00318-3

  Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

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  Sample test of MP00318-3

  The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

83291-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test HO-1/Hmox1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HO-1/Hmox1 fusion protein Eg0938
• Vollständiger Name: heme oxygenase (decycling) 1
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_010442.2
• Gene symbol: HO-1
• Gene ID (NCBI): 15368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.