HO-1/Hmox1 Rekombinanter Antikörper

HO-1/Hmox1 Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240236G4

Kat-Nr. : 83291-3-PBS

Synonyme

Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1

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  WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83291-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

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Geprüfte Anwendungen

Produktinformation

83291-3-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA HO-1/Hmox1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HO-1/Hmox1 fusion protein Eg0938
• Vollständiger Name: heme oxygenase (decycling) 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_010442.2
• Gene symbol: HO-1
• Gene ID (NCBI): 15368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.