HOMER1 Polyklonaler Antikörper

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HOMER1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12433-1-PBS

Synonyme

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• 

  WB analysis of rat brain using 12433-1-AP (same clone as 12433-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:5000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human/mouse brain using 12433-1-AP (same clone as 12433-1-PBS)

  WB result of anti-HOMER1 (12433-1-ap) with mouse brain lysate and human brain lysate. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 12433-1-AP (same clone as 12433-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C2C12 using 12433-1-AP (same clone as 12433-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12433-1-AP (same clone as 12433-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12433-1-AP (HOMER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IP experiment of fetal human brain using 12433-1-AP (same clone as 12433-1-PBS)

  IP result of anti-HOMER1 (IP:12433-1-AP, 4ug; Detection:12433-1-AP 1:1000) with fetal human brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12433-1-AP (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 12433-1-AP (same clone as 12433-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12433-1-AP (HOMER1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 12433-1-AP (same clone as 12433-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 12433-1-AP (same clone as 12433-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using HOMER1 antibody (12433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12433-1-AP (same clone as 12433-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12433-1-AP (HOMER1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 12433-1-AP (same clone as 12433-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human HOMER1 (12433-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12433-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12433-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA HOMER1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HOMER1 fusion protein Ag3109
• Vollständiger Name: homer homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 46-48 kDa
• GenBank-Zugangsnummer: BC015502
• Gene symbol: HOMER1
• Gene ID (NCBI): 9456
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.